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Figure 1
The nebnext chip seq library prep master mix set for illumina contains reagents for preparation of libraries for next generation sequencing on the illumina platform from 10 ng of chip dna in a standard workflow.
Chip seq library preparation. Preparation methods for chromatin immunoprecipitation chip seq dna libraries are similar to those for standard dna. However the amount of input dna for chip seq libraries is often lower than for standard dna library construction. Bioo scientific nextflex chip for the illumina platform. See genohub s up to date list of available illumina chip seq library prep services or if you re doing your own library preparation see the list of facilities that offer illumina sequencing.
In the regular chip seq although 10ng dna is a lot to a specific gene the amoount of dna is still very low. Each of these components must pass rigorous quality control standards and are lot controlled both individually and as a set of reagents. In my case the amount of dna for library preparation is low but to a specific gene. What quantity of chip dna is recommended for library preparation.
However the amount of input dna for chip seq libraries is lower than for standard dna library construction and library preparation requires the use of optimized reagents and protocols. Truseq chip library preparation kits are compatible with all illumina sequencers. The chip seq library prep reagent set for illumina contains enzymes and buffers that are ideally suited for sample preparation for next generation sequencing and for preparation of expression libraries. High library conversion efficiency.
Input dna amount from 5 ng to 30 ng. A quantity of 1 10 ng of chip dna is recommended for constructing a library with the nebnext chip seq library prep kit. Chip seq library prep fast total time 1 5 hrs. If you have less than 1 ng consider pooling replicate chip samples together in a maximum volume of 30 µl.
Preparation methods for chomatin immunoprecipitated chip seq dna libraries are similar to those for standard dna.
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